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recombinant hfhr5  (R&D Systems)
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R&D Systems recombinant hfhr5
Fig. 3 Factor H-related protein 5 wild-type and mutant proteins in cell supernatants and lysates. Factor H-related protein 5 was assayed by immunoblotting in transfected cell supernatants and lysates con- taining the wild-type sequence and the mutant M514R variant. Wild- type supernatant exhibited a strong band corresponding to FHR5. The supernatant from cells transfected with the CFHR5 M514R variant exhibited a very weak band. Control supernatants from HEK cells that were not transfected did not exhibit a band corresponding to FHR5. The FHR5 band was stronger in the lysates of the cells containing the M514R mutant variant compared to the wild-type, as the mutant variant was minimally secreted. <t>hFHR5,</t> recombinant human factor H-related protein 5; WT, wild-type; M514R, transfected mutant variant
Recombinant Hfhr5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3 Factor H-related protein 5 wild-type and mutant proteins in cell supernatants and lysates. Factor H-related protein 5 was assayed by immunoblotting in transfected cell supernatants and lysates con- taining the wild-type sequence and the mutant M514R variant. Wild- type supernatant exhibited a strong band corresponding to FHR5. The supernatant from cells transfected with the CFHR5 M514R variant exhibited a very weak band. Control supernatants from HEK cells that were not transfected did not exhibit a band corresponding to FHR5. The FHR5 band was stronger in the lysates of the cells containing the M514R mutant variant compared to the wild-type, as the mutant variant was minimally secreted. hFHR5, recombinant human factor H-related protein 5; WT, wild-type; M514R, transfected mutant variant

Journal: Pediatric nephrology (Berlin, Germany)

Article Title: Complement dysregulation associated with a genetic variant in factor H-related protein 5 in atypical hemolytic uremic syndrome.

doi: 10.1007/s00467-023-06184-6

Figure Lengend Snippet: Fig. 3 Factor H-related protein 5 wild-type and mutant proteins in cell supernatants and lysates. Factor H-related protein 5 was assayed by immunoblotting in transfected cell supernatants and lysates con- taining the wild-type sequence and the mutant M514R variant. Wild- type supernatant exhibited a strong band corresponding to FHR5. The supernatant from cells transfected with the CFHR5 M514R variant exhibited a very weak band. Control supernatants from HEK cells that were not transfected did not exhibit a band corresponding to FHR5. The FHR5 band was stronger in the lysates of the cells containing the M514R mutant variant compared to the wild-type, as the mutant variant was minimally secreted. hFHR5, recombinant human factor H-related protein 5; WT, wild-type; M514R, transfected mutant variant

Article Snippet: Recombinant hFHR5 was used as the standard (catalog number: 3845-F5, R&D Systems).

Techniques: Mutagenesis, Western Blot, Transfection, Sequencing, Variant Assay, Control, Recombinant